Projects

AMPAR and GABAR

Insertion 

 

Neurotransmitter receptor insertion and removal to and from synapsis underlie many forms of experience-dependent plasticity including learning and memory. We plan to use in-vivo molecular manipulations to study receptor trafficking within specific neuronal circuits.

LDCVs Exocytosis

 

Large dense core vesicles (LDCVs)  have been proposed to release neuroactive subtances such as neuropeptides and neurotrophic factors. We focus on elucidating the  fusion machinery of  LDCVs exocytosis and their role in regulating synaptic transmission and neuronal circuit formation and refinement.

Recent Publications

Mapping Circuit Neurodegeneration

 

Using novel targeted viral and genetic manipulations, we will interrogate the adaptations of neuronal circuits affected early during neurodegenerative diseases susc as Alzheimer's Disease. Analyzing the reorganization of circuits represents an exciting new direction in understanding normal and pathological aging of the brain.

 

 

 

 

New Strategies for Regulating Membrane Dynamics and Neuronal Networks

 

Another goal of the lab is to develop novel molecular  tools to regulate neuronal signaling and function. We are interested in exploring optogentic tools and photoactivable molecules to regulate membrane dynamics such as endo and exocytosis in-vivo and in-vitro

preparations such as cultured neurons.

 

 

 

 

  • Madrigal MP, Portales A, Perez Sanjuan M, Jurado S (2018) Postsynaptic SNARE proteins: Role in synaptic transmission and plasticity. Neuroscience. pii: S0306-4522(18)30736-X. doi: 10.1016/j.neuroscience.2018.11.012. 

  • Jurado S (2018) AMPA Receptor Trafficking in Natural and Pathological Aging. Front Mol Neurosci. 10:446. doi: 10.3389/fnmol.2017.00446.

  • Ramirez-Franco JJ, Munoz-Cuevas FJ, Lujan R, Y, Jurado S (2016) Excitatory and Inhibitory Neurons in the Hippocampus Exhibit Molecularly Distinct Large Dense Core Vesicles. Frontiers in Cellular Neuroscience. 10:202. doi: 10.3389/fncel.2016.00202. 

 

  • Bacaj T*, Ahmad M*, Jurado S*, Malenka RC, Südhof TC (2015) Synaptic function of Rab11Fip5: Selective Requirement for Hippocampal Long-Term Depression. J. Neurosci. 35(19):7460-74.

 

  • Arendt KL, Zhang Y, Jurado S, Malenka RC, Südhof TC, Chen L (2015) Retinoic Acid and LTP Recruit Postsynaptic AMPA Receptors Using Distinct SNARE-Dependent Mechanisms. Neuron 86(2): 442-56.

 

  • Kristian PK, Quach LN, Solé M, Axtell RC, Nguyen TV, Soler-Llavina GJ, Jurado S, Han J, Steinman L, Longo FM, Schneider JA, Malenka RC, Buckwalter MS. (2015) B-Lymphocyte-Mediated Delayed Cognitive Impairment following Stroke. J Neurosci. 35(5):2133-45.

 

  • Jurado S (2014) The dendritic SNARE fusion machinery involved in AMPARs insertion during long-term potentiation. Frontiers in Cellular Neuroscience. 8:407. doi: 10.3389/fncel.2014.00407.

 

  • Schwartz N, Temkin P, Jurado S, Lim BK, Heifets BD, Polepalli JS, Malenka RC. Decreased motivation during chronic pain requires long-term depression in the nucleus accumbes. Science 2014 345(6196):535-42.

 

  • Jurado S*, Goswami D*, Zhang Y, Südhof TC, Malenka RC. LTP requires unique postsynaptic SNARE fusion machinery. Neuron 2013 77(3):542-58.

 

  • Jurado S, Knafo S. Microscale AMPAR Reorganization and Dynamics of the Postsynaptic Density. J Neurosci. 2012 23(32): 7103-5.

 

  • Jurado S, Biou V, Malenka RC. A calcineurin/AKAP complex is required for NMDA receptor dependent-LTD. Nat. Neurosci. 2010 13(9):1053-5.

 

  • Jurado S, Benoist M, Lario A, Petrok CM, Esteban JA. PTEN is recruited to the postsynaptic terminal for NMDA receptor-dependent long-term depression. EMBO J. 2010 29(16):2827-40.